目的 比较大孔树脂法与醇沉法对小叶榕水提液中荭草苷、异荭草苷、牡荆苷和异牡荆苷4个黄酮类成分吸收特征的影响。方法 通过大鼠肠外翻模型,以累积吸收量(Q)与吸收速率常数(Ka)为指标,采用HPLC-PDA测定不同药物质量浓度(8、16、32 mg·mL-1)下肠吸收液样品中荭草苷、异荭草苷、牡荆苷和异牡荆苷4个黄酮类成分在十二指肠、空肠、回肠和结肠中的吸收情况。结果 在不同给药浓度下,4个化学成分的肠吸收均为线性吸收(r2>0.9),符合一级吸收速率;吸收速率常数与给药浓度、肠道部位有关;质量浓度相同的大孔树脂和醇沉纯化物溶液,4个成分在小肠段的吸收速率常数和累计吸收量,均为大孔树脂组高于醇沉组。结论 从外翻肠囊法的角度而言,大孔树脂纯化工艺更有利于小叶榕药效物质的吸收。
Abstract
OBJECTIVE To compare the effect of macroporous resin method and alcohol precipitation method on the absorption characteristics of four flavonoids components of orientin, isoorientin, vitexin and isovitrin in water extract of Ficus microcarpa. METHODS Using the intestinal valgus model of rats and cumulative absorption Q and yield rate constant Ka as indicators, HPLC-PDA method was used to determine the absorption of four flavonoids, orientin, isoorientin, vitexin and isovitrin in duodenum, jejunum, ileum and colon in the intestinal absorption samples of different drug concentrations. RESULTS At different administration concentrations, the intestinal absorption of the four chemical components was linear absorption (r2>0.9), which was consistent with the zero-order absorption rate; the absorption rate constant was related to the administration concentration and intestinal site; the mass concentration was same absorption rate constant and cumulative absorption of the four components in the pore resin and alcohol precipitation purified product in the intestine segment were higher in the macroporous resin group than in the alcohol precipitation group. CONCLUSION The D101 macroporous resin purification process is suitable for the purification of ficus lobata water extract, and is more conducive to the absorption of orientin, isoorientin, vitexin, and isovitrin in the intestine.
关键词
小叶榕干浸膏 /
外翻肠囊法 /
累积吸收量 /
吸收速率常数
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Key words
dry extract of Ficus microcarpa /
everted intestinal sac method /
cumulative absorption /
absorption rate constant
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中图分类号：
R965
R944
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参考文献
[1] YUAN R, SUN L L, GUAN S Y, et al. HPLC fingerprint and LC-Q-TOF-MS analysis of Xiaoyerong dry extract and Keteling capsule [J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2016, 22(3): 57-62.
[2] JING C L. Extraction, purification and antioxidant activity of total flavonoids from alfalfa [D]. Beijing:Chinese Academy of Agricultural Sciences, 2016.
[3] XU Q, MA CHAO M R, WEI W, et al. Determination of 12 components in banyan dry extract of Keteling by UPLC-QQQMS [J]. J Guangxi Normal Univ (广西师范大学学报), 2017, 35 (2): 80-86.
[4] LIU L H, WANG L S, WANG T T, et al. Research progress on chemical constituents and pharmacological activities of Ficus microcarpa [J]. Lishizhen Med Mater Med Res(时珍国医国药), 2008, 19(2): 390-392.
[5] LONG G J, HUANG Y, JIANG L H, et al. The total flavanone of Ficus microcarpa L. leaf withdraws and distinction [J]. Lishizhen Med Mater Med Res(时珍国医国药), 2006, 17(8):1425-1426.
[6] Ch.P(2015) Vol Ⅰ(中国药典2015年版.一部)[S]. 2015.
[7] LIU L H, WANG L S, FENG D D, et al. Adsorption and separation of total flavonoids from Ficus microcarpa leaves by macroporous resin [J]. Fine Chem Ind(精细化工), 2009, 26 (3): 293-298.
[8] LIU Y, TANG L, CAO X, et al. Absorption of extractive Polygonum orientale in rat everted gut sacs[J]. China J Tradit Chin Med(中国中药杂志), 2014, 39 (11): 2121-2125.
[9] WEI J B, HUANG R B, LIN J, et al. Comparative study on antitussive and antiasthmatic effects of aqueous extract and ethanol extract of Ficus microcarpa [J]. Guangxi Tradit Chin Med(广西中医药), 2006, 29 (4): 58-59.
[10] FANG D S. Anti inflammatory and analgesic effects of total flavonoids from Ficus microcarpa leaves [J]. J China Pharm(中国药房), 2012, 23(39): 3673-3675.
[11] WANG R F, GENG D, WU X W, et al. Anti inflammatory activity of four main components in Trollius chinensis [J]. Lishizhen Med Mater Med Res(时珍国医国药), 2012, 23 (9): 2115-2116.
[12] ZHU X Z, SHEN W W, GONG C Y, et al. Study on the anti respiratory syncytial virus activity of isoorientin in vitro and in vivo [J]. J Sun Yatsen Univ(中山大学学报), 2015, 36 (3): 352-359.
[13] ZHANG H, AN R, XU R C, et al. Study on the eversion absorption of flavonoids in Gegen Qinlian Decoction and different compatibility groups [J]. Chin J Tradit Chin Med(中国中药杂志), 2011, 36 (23): 3332-3337.
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脚注
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基金
中国东盟传统药物研究国际合作联合实验室建设项目资助(一期)(桂科AD17195025);广西重点研发计划项目资助(桂科AB17292048)
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